Date Thesis Awarded

5-2010

Document Type

Honors Thesis

Degree Name

Bachelors of Science (BS)

Department

Biology

Advisor

Patty Zwollo

Committee Member

Mark H. Forsyth

Committee Member

Matthew J. Wawersik

Committee Member

Louis Rossiter

Abstract

Rainbow trout are farmed worldwide as a popular food source, and represent a critical stage in the evolutionary history of the immune system. Teleosts lack bone marrow, the site of hematopoiesis in mammals. Instead, the anterior kidney of many fish species has been described as the site of early B cell development. This project sought to expand on a previously published model of the B cell populations in trout immune tissues using flow cytometry analysis of the kidney. The model suggests that a maturation gradient exists along the trout kidney, with the earliest B cell precursors in the anterior kidney, and mature B cells residing in the posterior kidney. If the maturation gradient exists, this project hypothesized that detecting B cell populations in the kidney would reveal an inverse relationship between precursor and mature B cell populations along the kidney gradient. Cell size, cell complexity, and antibodies which detect the transcription factors EBF, Pax5, and Xbp-1, as well as anti-IgM and RAG-1 antibodies were used to define precursor and mature B cell populations throughout the kidney of small and large trout. This project confirmed an inverse relationship between the precursor and mature B cell populations along the kidney gradient in large fish, with specialization of the anterior kidney for early B cell development, and the posterior kidney containing many resting mature B cells. Differences were also discovered between large and small fish mature B cell populations and Xbp-1 expression.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

Comments

Thesis is part of Honors ETD pilot project, 2008-2013. Migrated from Dspace in 2016.

Share

COinS